LymphedemaV1, Main, Exploration, bibRecord, 003981

In vitro self-assembly of human pericyte-supported endothelial microvessels in three-dimensional co-culture: a simple model for interrogating endothelial:pericyte interactions

Identifieur interne : 003981 ( Main/Exploration ); précédent : 003980; suivant : 003982

In vitro self-assembly of human pericyte-supported endothelial microvessels in three-dimensional co-culture: a simple model for interrogating endothelial:pericyte interactions

Auteurs : Jp Waters [Royaume-Uni] ; Ms Kluger [États-Unis] ; M. Graham [États-Unis] ; Wg Chang [États-Unis] ; Jr Bradley [Royaume-Uni] ; Js Pober [États-Unis]

Source :

Journal of vascular research [ 1018-1172 ] ; 2013.

RBID : PMC:3879598

Abstract

We describe a method for co-culture of macro or microvascular human endothelial cells (ECs) and pericytes (PCs) within a 3-dimensional (3-D) protein matrix resulting in lumenized EC cords invested by PCs. To prevent apoptotic cell death of ECs in 3-D culture, human umbilical vein or dermal microvascular ECs were transduced to express the anti-apoptotic protein Bcl-2. To prevent PC-mediated gel contraction, the collagen-fibronectin gel was polymerized within a polyglycolic acid (PGA) non-woven matrix. Over the first 24-48 h, EC-only gels spontaneously formed cords that developed lumens via vacuolization; such vascular networks were maintained for up to 7 days. In EC-PC co-cultures, PCs were recruited to the EC networks. PC investment of EC cords both limited the lumen diameter and increased the degree of vascular network arborization. Peg and socket junctions formed between ECs and PCs in this system but dye transfer, indicative of gap junction formation, was not observed. This simple system can be used to analyze bidirectional signals between ECs and PCs in a 3-D geometry.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3879598

DOI: 10.1159/000353303
PubMed: 23860328
PubMed Central: 3879598

Affiliations:

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en"><italic>In vitro</italic>
 self-assembly of human pericyte-supported endothelial microvessels in three-dimensional co-culture: a simple model for interrogating endothelial:pericyte interactions</title>
<author><name sortKey="Waters, Jp" sort="Waters, Jp" uniqKey="Waters J" first="Jp" last="Waters">Jp Waters</name>
<affiliation wicri:level="4"><nlm:aff id="A3">Department of Medicine, University of Cambridge, Addenbrooke’s Hospital, Hills Road, Cambridge, CB2 0QQ, UK</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Department of Medicine, University of Cambridge, Addenbrooke’s Hospital, Hills Road, Cambridge, CB2 0QQ</wicri:regionArea>
<orgName type="university">Université de Cambridge</orgName>
<placeName><settlement type="city">Cambridge</settlement>
<region type="country">Angleterre</region>
<region type="région" nuts="1">Angleterre de l'Est</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kluger, Ms" sort="Kluger, Ms" uniqKey="Kluger M" first="Ms" last="Kluger">Ms Kluger</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Department of Immunobiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Immunobiology, Yale University School of Medicine, New Haven, Connecticut 06520</wicri:regionArea>
<wicri:noRegion>Connecticut 06520</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Graham, M" sort="Graham, M" uniqKey="Graham M" first="M" last="Graham">M. Graham</name>
<affiliation wicri:level="1"><nlm:aff id="A2">Center for Cellular and Molecular Imaging, Yale University School of Medicine, New Haven, Connecticut 06520, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Center for Cellular and Molecular Imaging, Yale University School of Medicine, New Haven, Connecticut 06520</wicri:regionArea>
<wicri:noRegion>Connecticut 06520</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chang, Wg" sort="Chang, Wg" uniqKey="Chang W" first="Wg" last="Chang">Wg Chang</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Department of Immunobiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Immunobiology, Yale University School of Medicine, New Haven, Connecticut 06520</wicri:regionArea>
<wicri:noRegion>Connecticut 06520</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Bradley, Jr" sort="Bradley, Jr" uniqKey="Bradley J" first="Jr" last="Bradley">Jr Bradley</name>
<affiliation wicri:level="4"><nlm:aff id="A3">Department of Medicine, University of Cambridge, Addenbrooke’s Hospital, Hills Road, Cambridge, CB2 0QQ, UK</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Department of Medicine, University of Cambridge, Addenbrooke’s Hospital, Hills Road, Cambridge, CB2 0QQ</wicri:regionArea>
<orgName type="university">Université de Cambridge</orgName>
<placeName><settlement type="city">Cambridge</settlement>
<region type="country">Angleterre</region>
<region type="région" nuts="1">Angleterre de l'Est</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Pober, Js" sort="Pober, Js" uniqKey="Pober J" first="Js" last="Pober">Js Pober</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Department of Immunobiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Immunobiology, Yale University School of Medicine, New Haven, Connecticut 06520</wicri:regionArea>
<wicri:noRegion>Connecticut 06520</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">23860328</idno>
<idno type="pmc">3879598</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3879598</idno>
<idno type="RBID">PMC:3879598</idno>
<idno type="doi">10.1159/000353303</idno>
<date when="2013">2013</date>
<idno type="wicri:Area/Pmc/Corpus">003922</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">003922</idno>
<idno type="wicri:Area/Pmc/Curation">003921</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">003921</idno>
<idno type="wicri:Area/Pmc/Checkpoint">002579</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">002579</idno>
<idno type="wicri:Area/Ncbi/Merge">005B38</idno>
<idno type="wicri:Area/Ncbi/Curation">005B38</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">005B38</idno>
<idno type="wicri:Area/Main/Merge">003990</idno>
<idno type="wicri:Area/Main/Curation">003981</idno>
<idno type="wicri:Area/Main/Exploration">003981</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main"><italic>In vitro</italic>
 self-assembly of human pericyte-supported endothelial microvessels in three-dimensional co-culture: a simple model for interrogating endothelial:pericyte interactions</title>
<author><name sortKey="Waters, Jp" sort="Waters, Jp" uniqKey="Waters J" first="Jp" last="Waters">Jp Waters</name>
<affiliation wicri:level="4"><nlm:aff id="A3">Department of Medicine, University of Cambridge, Addenbrooke’s Hospital, Hills Road, Cambridge, CB2 0QQ, UK</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Department of Medicine, University of Cambridge, Addenbrooke’s Hospital, Hills Road, Cambridge, CB2 0QQ</wicri:regionArea>
<orgName type="university">Université de Cambridge</orgName>
<placeName><settlement type="city">Cambridge</settlement>
<region type="country">Angleterre</region>
<region type="région" nuts="1">Angleterre de l'Est</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kluger, Ms" sort="Kluger, Ms" uniqKey="Kluger M" first="Ms" last="Kluger">Ms Kluger</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Department of Immunobiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Immunobiology, Yale University School of Medicine, New Haven, Connecticut 06520</wicri:regionArea>
<wicri:noRegion>Connecticut 06520</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Graham, M" sort="Graham, M" uniqKey="Graham M" first="M" last="Graham">M. Graham</name>
<affiliation wicri:level="1"><nlm:aff id="A2">Center for Cellular and Molecular Imaging, Yale University School of Medicine, New Haven, Connecticut 06520, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Center for Cellular and Molecular Imaging, Yale University School of Medicine, New Haven, Connecticut 06520</wicri:regionArea>
<wicri:noRegion>Connecticut 06520</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chang, Wg" sort="Chang, Wg" uniqKey="Chang W" first="Wg" last="Chang">Wg Chang</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Department of Immunobiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Immunobiology, Yale University School of Medicine, New Haven, Connecticut 06520</wicri:regionArea>
<wicri:noRegion>Connecticut 06520</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Bradley, Jr" sort="Bradley, Jr" uniqKey="Bradley J" first="Jr" last="Bradley">Jr Bradley</name>
<affiliation wicri:level="4"><nlm:aff id="A3">Department of Medicine, University of Cambridge, Addenbrooke’s Hospital, Hills Road, Cambridge, CB2 0QQ, UK</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Department of Medicine, University of Cambridge, Addenbrooke’s Hospital, Hills Road, Cambridge, CB2 0QQ</wicri:regionArea>
<orgName type="university">Université de Cambridge</orgName>
<placeName><settlement type="city">Cambridge</settlement>
<region type="country">Angleterre</region>
<region type="région" nuts="1">Angleterre de l'Est</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Pober, Js" sort="Pober, Js" uniqKey="Pober J" first="Js" last="Pober">Js Pober</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Department of Immunobiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Immunobiology, Yale University School of Medicine, New Haven, Connecticut 06520</wicri:regionArea>
<wicri:noRegion>Connecticut 06520</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">Journal of vascular research</title>
<idno type="ISSN">1018-1172</idno>
<idno type="eISSN">1423-0135</idno>
<imprint><date when="2013">2013</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p id="P1">We describe a method for co-culture of macro or microvascular human endothelial cells (ECs) and pericytes (PCs) within a 3-dimensional (3-D) protein matrix resulting in lumenized EC cords invested by PCs. To prevent apoptotic cell death of ECs in 3-D culture, human umbilical vein or dermal microvascular ECs were transduced to express the anti-apoptotic protein Bcl-2. To prevent PC-mediated gel contraction, the collagen-fibronectin gel was polymerized within a polyglycolic acid (PGA) non-woven matrix. Over the first 24-48 h, EC-only gels spontaneously formed cords that developed lumens via vacuolization; such vascular networks were maintained for up to 7 days. In EC-PC co-cultures, PCs were recruited to the EC networks. PC investment of EC cords both limited the lumen diameter and increased the degree of vascular network arborization. Peg and socket junctions formed between ECs and PCs in this system but dye transfer, indicative of gap junction formation, was not observed. This simple system can be used to analyze bidirectional signals between ECs and PCs in a 3-D geometry.</p>
</div>
</front>
</TEI>
<affiliations><list><country><li>Royaume-Uni</li>
<li>États-Unis</li>
</country>
<region><li>Angleterre</li>
<li>Angleterre de l'Est</li>
</region>
<settlement><li>Cambridge</li>
</settlement>
<orgName><li>Université de Cambridge</li>
</orgName>
</list>
<tree><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Waters, Jp" sort="Waters, Jp" uniqKey="Waters J" first="Jp" last="Waters">Jp Waters</name>
</region>
<name sortKey="Bradley, Jr" sort="Bradley, Jr" uniqKey="Bradley J" first="Jr" last="Bradley">Jr Bradley</name>
</country>
<country name="États-Unis"><noRegion><name sortKey="Kluger, Ms" sort="Kluger, Ms" uniqKey="Kluger M" first="Ms" last="Kluger">Ms Kluger</name>
</noRegion>
<name sortKey="Chang, Wg" sort="Chang, Wg" uniqKey="Chang W" first="Wg" last="Chang">Wg Chang</name>
<name sortKey="Graham, M" sort="Graham, M" uniqKey="Graham M" first="M" last="Graham">M. Graham</name>
<name sortKey="Pober, Js" sort="Pober, Js" uniqKey="Pober J" first="Js" last="Pober">Js Pober</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003981 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003981 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Sante
   |area=    LymphedemaV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     PMC:3879598
   |texte=   In vitro self-assembly of human pericyte-supported endothelial microvessels in three-dimensional co-culture: a simple model for interrogating endothelial:pericyte interactions
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:23860328" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a LymphedemaV1

This area was generated with Dilib version V0.6.31.
Data generation: Sat Nov 4 17:40:35 2017. Site generation: Tue Feb 13 16:42:16 2024

Serveur d'exploration sur le lymphœdème

In vitro self-assembly of human pericyte-supported endothelial microvessels in three-dimensional co-culture: a simple model for interrogating endothelial:pericyte interactions

In vitro self-assembly of human pericyte-supported endothelial microvessels in three-dimensional co-culture: a simple model for interrogating endothelial:pericyte interactions

Source :

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration sur le lymphœdème
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.